You wish to develop a monoclonal antibody (mAb) that binds with high affinity to the SARS-CoV-2 spike (S) protein, for use as a therapeutic agent. You have isolated a human B-cell clone that produces an IgG that is specific for the SARS-CoV-2 S protein, but its affinity for binding the target epitope is low. Describe in conceptual detail how you could use a protein engineering approach to enhance the affinity of the mAb for the target antigen.​

you did not attach the discourse, but i assume it to be the discourse on the origin and basis of inequality among men (the original titile in french is: "discours sur l'origine et les fondements de l'inégalité parmi les hommes"), also know as "second discourse". in this discourse, jean-jacques rousseau emphasizes that private property is the basis of all inequalities.

therefore, what enabled the old regime to maintain as long as it did was private property.

osteogenesis imperfecta (oi) refers to a congenital ailment of bones. in the condition, the bones become brittle or fragile. this ailment is a general result of the defect or mutations in the genes (colia1 or colia2). this mutation results in imperfect collagen formation either in quantity or quality wise.  

there are eight kinds of oi defects, and each kind varies with certain distinctions in symptoms. the usual symptoms include frequent bone fractures, loss of hearing in infants, protruding eyes, discoloration of the white in the eyes, respiratory issues, loosening of the joints, short stature, and other symptoms.  

garbage disposal because lysosomes dispose of old cell parts like garbage disposals dispose of old food.