Allosteric effectors: Question 1 options: can lead to a decrease in the availability of a protien alter enzyme activity by binding to the active site of an enzyme can cause large changes in enzymatic activity decrease the sensitivity of the enzyme at nearly all concentrations of substrate do not alter the sensitivity of a metabolic pathway

can cause large changes in enzymatic activity

Explanation:

An enzyme refers to a biological catalyst that is typically used to speed up (accelerate) the rate of a chemical reaction by lowering the activation energy of its reactants.

An allosteric effector can be defined as an agent, organ or molecule that is being binded to an enzyme at a site, thereby causing a reduction (negative effect) or an increase (positive effect) in an enzyme activity.

When the activation energy of a reaction is low, the rate of the reaction would be faster. Therefore, an enzyme speeds or catalyzes the rate of a reaction by lowering its activation energy.

Additionally, if the conditions are not optimal for an enzyme, it limits the ability of an enzyme to bind or be joined with its substrates.

Furthermore, an increase in temperature increases or speeds up the rate of a reaction while low temperature limits or reduces the rate of a reaction.

In the human body, the optimal temperature for enzymes is around 37 degrees celsius (°C).

In conclusion, an allosteric effector can cause large changes in enzymatic activity because it acts as an intermediary and mediates specific effect in a metabolic pathway.

this protocol describes a detailed procedure for intracellular flow cytometry staining.