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One of the procedures for cloning foreign DNA segments takes advantage of restriction endonucleases such as HindIII that produce complementary single strand ends (sticky ends). These enzymes produce identical sticky ends on cleaved foreign DNA and on the vector DNA into which you insert your DNA. Assume you have inserted your favorite gene into the HindIII site of the Bluescript cloning vector with ligase, you amplified it in E. coli, and isolated a large quantity of the plasmid with the insert. How do you excise your favorite gene from the plasmid?
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One of the procedures for cloning foreign DNA segments takes advantage of restriction endonucleases...
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