Arrange the following statements in chronological order.

The bacterium is used to introduce the Bt toxin gene into the chromosomes of plant cells.
The gene for Bt toxin is isolated from the bacterium and inserted into the plasmid
The plasmid is taken up by bacterium
Ti plasmid is isolated and prepared for the insertion of foreign DNA
a single plant cell is chosen to produce a plant that now produces Bt toxin
1. Ti plasmid is isolated and prepared for the insertion of foreign DNA
2. the gene for Bt toxin is isolated from the bacterium and inserted into the plasmid
3. the plasmid is taken up by bacterium
4. the bacterium is used to introduce the Bt toxin gene into the chromosomes of plant cells.
5. a single plant cell is chosen to produce a plant that now produces Bt toxin

How do restriction enzymes cut DNA sequences?

a. They have the ability to cut DNA randomly.
b. They cut DNA at sites, called recognition sites, that have specific nucleotide sequences.
c. They cut DNA at sequences that have lots of adenine bases.