You decide to design an assay for flu infection detection. ha protein is only present on the surface of influenza viral particles so you choose to design an elisa assay to detect the presence of ha in human blood samples.

you will use three different antibodies for the assay. the experimental procedure is as follows:

step 1. incubate your assay plate with antibody (i) solution overnight to coat the surface of the assay plate with antibody (i). remove antibody (i) solution and wash the plate extensively.

step 2. add blood samples to the coated plate and incubate for 30 min. remove blood sample and wash the plate extensively.

step 3. add antibody (ii) solution to the plate and incubate for 30 min. remove antibody (ii) solution and wash the plate extensively.

step 4. add antibody (iii) solution to the plate and incubate for 30 min. remove antibody (iii) solution and wash the plate extensively.

step 5. add tmb substrate to the plate and monitor color development.

What would be an example of hypothesis for goldfish respiration lab

The tasmanian devil, a marsupial carnivore, is facing extinction due to devil facial tumor disease (dftd) which causes bulging cancerous lumps and lesions to erupt around the face and neck — often causing enough deformation to make seeing or eating difficult. dftd has evolved into a contagious cancer, a trait that is unique among cancers. devil mating behavior involves biting around the head and neck, allowing cells from one individual — especially cells from the crumbly dftd tumors — to be transferred to the wounds or face of a new individual. this marsupial was once found across australia, but sea levels rose, isolating the tasmanian population, while the australian population went extinct. what would be an outcome of genetic isolation that is likely to have impacted the spread of dftd? a) reduced territory puts diseased individuals in greater contact with non-diseased ones. b) inbreeding results in less variation in facial features so the cancer is generally fatal. c) genetic isolation has made it difficult for scientists to develop a vaccine against dftd. d) the lack of genetic variation in the immune system of tasmanian devils minimizes resistance to the disease.

Photosynthesis uses co2 and cellular respiration produces co2. we call the point when the two processes are in balance--when there is no net production of co2--the compensation point. how might you limit one of the processes in order to achieve a compensation point?