Identify the plasmid that must contain the cd3γ core promoter sequence but the fewest or no negative regulatory sequences. Based on the data in figure 2, describe the most likely cause of the variation in luciferase activity among the cells that contain plasmids pcd3γ-419 , pcd3γ-309 , pcd3γ-239 , and pcd3γ-199. Calculate the approximate percent increase in luciferase activity between cells containing plasmid pcd3γ-59 and cells containing plasmid pcd3γ-149. Round to the nearest whole number

ANSWER:

-The response indicates that the plasmid that must contain the CD3γ core promoter sequence but the fewest or no negative regulatory sequences is pCD3γ-15 (because this plasmid gives the greatest luciferase activity).

-The response indicates that the different sequences cloned into the plasmids contain different combinations of positive and negative regulatory sequences.

-The response indicates that the percent increase is approximately 100%. (The pCD3γ-59 bar ends at the 2 and the pCD3γ-149 bar ends at the 4 on the axis labeled Relative Luciferase Activity.)

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