Physics, 09.04.2021 02:40 yousifgorgees101
In the process of fluorescence, a molecule in its ground state will absorb a photon with a certain energy Eex, called the excitation energy, and then emit a photon with energy Eem, the emission energy. Obviously, the molecule cannot emit more energy than was absorbed.
a) You wrote in your notebook that you excited a fluorescent bead at λ = 640 nm and found that it fluoresced (emitted) at λ = 520 nm. Or was it the other way around? Do a calculation and see if those figures should be switched.
b) You shine a laser on your bead at the excitation wavelength. The laser has a power of 1 mW. How many photons are emitted by the laser in one second?
c) You wish to build a microscope that allows you to excite the bead at its excitation wavelength and detect the fluorescence at the emission wavelength. This requires a pair of filters, one that allows only the excitation wavelength to pass through, and the other that allows only the emission wavelength to pass through. What color will these filters appear (i. e. what color light will they transmit?) You may have to look at an electromagnetic spectrum to figure this out…
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