A sample with a path length of 1 cm absorbs 99.0% of the incident light at a wavelength of 274 nm, measured with respect to an appropriate solvent blank. Tyrosine is known to be the only chromophore present in the sample that has significant absorption at 274 nm. Calculate the molar concentration of tyrosine in the sample.

17. NADH has a molar extinction coefficient of 6200 M2 cm at 340 nm. Calculate the molar concentration of NADH required to obtain an absorbance of 0.1 at 340 nm in a 1-cm path length cuvette. 18. A sample with a path length of 1 cm absorbs 99.0% of the incident light at a wavelength of 274 nm, measured with respect to an appropriate solvent blank. Tyrosine is known to be the only chromophore present in the sample that has significant absorption at 274 nm. Calculate the molar concentration of tyrosine in the sample.

Explanation:

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