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When conducting an experiment to determine what effect the temperature of water has on the solubility of sugar, what variable(s) should remain constant?

I. The amount of water used.
II. The temperature of the water.
III. The type of sugar used.
IV. The surface area of the sugar.
V. The agitation applied to the solution.

A. I, III, IV, and V
B. All of these
C. I and III only
D. II only