Determine the micromolar (µm) extinction coefficient (e; units are µm-1 cm-1) for a protein solution assuming that it has a molecular weight of 55,000 g/mole. the slope of the standard curve is 0.973 ml/µg. convert the slope units of ml/µg to µm-1 cm-1.

Arigid tank is divided into two equal parts by a partition. one part of the tank contains 3 kg of compressed liquid water at 400 kpa and 60°c while the other part is evacuated. the partition is now removed, and the water expands to fill the entire tank. determine the entropy change of water during this process, if the final pressure in the tank is 40 kpa. use steam tables.