Add fluo-3AM (lipid soluble) Cleave fluo-3AM to fluo-3 (water soluble)
Increase in fluorescence when bound to calcium
Calcium gets into cell through ion channels and via phospholipase Cβ

Anurse recently administered filgrastim intravenously to a client who has cancer and is receiving cytotoxic chemotherapy. for which of the following data, discovered after the medication was administered, should the nurse consider filling an incident report? a. the client had chemotherapy 12 hr before the medication was administered. b. the nurse flushed the client’s iv line with d5w before and after the medication was administered. c. the client’s absolute neutrophil count was 2,500/mm^3 before the medication was administered. d. the medication vial sat at the room temperature for 2 hr before it was administered.