Astudent needed to prepare 500ml of 1x tae buffer to run a qc gel. the stock solution in the lab is 5x tae. what volumes of stock tae and water are needed to prepare the 1x working tae buffer?

Carla was trying to decide what to do saturday evening. she has $100 available to spend for the evening. she has narrowed her choices down to these events: braves game, a concert of an artist she likes, or the georgia aquarium. admission to the braves game is $25, hot dogs cost $10, sodas cost $5. the concert costs $25 for admission, $20 for a souvernir t-shirt, and $10 for a soda. the georgia aquarium has the show of a lifetime with an all you can eat and drink that costs $100. carla does not want to take any driving chances, so in addition to the cost of the aquarium, she will also need to pay for her uber/lyft, which is $15 each way. which option should carla choose if she wants to spend the least? based on that choice, what then is carla's oppotunity cost? fully explain your answer in at least one paragraph.

Which of the following circles have their centers in the third quadrant?

What is the measure of each of the two angles formed by the bisector of the diagonal of a rhombus if the original angle measures 58 degrees?