As colonist from the Lowcountry of Carolina move west, what new challenges did they face?

In Carolina's first fifty years, the colony faced violent rebellion, attack by the Spanish, war with Indians, hurricanes, droughts, and pirates. Despite all these problems, Carolina grew.

dirty, contaminated water

contaminated food,

chlostrophobic living spaces,

mice infestation,

etc,

genetics is the study of how dna works, the methodologies defining the new recent technologies allow scientist to have a panoramic view of the entire dna landscape, especially how genomes interact with their environment, including off course, factors outside their organisms.

thus is very possible the existence of both scenarios, where populations of isolated individuals have different genetic traits, and share traits related to genetic mutation, due rats can easily travel overseas using human transport channels. so scientists could get a vast number of data, including human interaction, evolution of rodents, health development, spread of infections, development of new organisms, how genomes evolve in different environments and so on.