As the United States acquired more land between 1803 and 1850, what issue significantly increased sectional tensions?

I would say Slavery

Explanation:

One example of this is the time after Louisiana Purchase in 1803. Missouri wanted statehood as a slave state. However, the Tallmadge Amendment was proposed where no slaves could be imported, and children born after Missouri's admission are free. Southerners disliked this and the Missouri Compromise was made to admit Missouri as a slave state and Maine as a free state to maintain the northern and southern power in the government.

There are so many times when Congress fought over slavery such as Bleeding Kansas and the Great Compromise of 1850.

i think it was   tsar alexander ii

1960: wilma rudolph was the first woman to win three olympic gold medals in track and field at one olympic games. she was an american sprinter from tennessee, she was acclaimed the fastest woman in the world. she was among the most visible black women in america and abroad. she became a role model for black and female athletes. she was also known as a civil rights and women right's pioneer.

1973: billy jean king is an american former number one tennis player. she represented the united states in the federal cup and the wightman cup. for three years she was the usa's captain in the federation cup. she defends gender equality and has been a pioneer for equal and social justice. at the age of 29, she won "the battle of sexes" tennis match against the 55 year old bobby riggs. she was also the founder of women's tennis association and the women's sports foundation.  

1983: tamara mckinney becomes the first american female skier to win the alpine world cup overall championship top. she was the only american to hold that title for a quarter century until 2008.

1992: manon rhéaume, from canada,   becomes the only woman to start in a national hockey league game in an exhibition game for the tampa bay lightning . she played on the canada women's national ice hockey team winning gold medals in 1992 and 1994. and silver medals in 1998. manon marked the first time a woman appeared in a regular season professional game with her first appearance against the salt lake golden eagles.

2013: serena williams won 78 of 82 matches at the age of 31. she is an american professional tennis player. she reached the number 1 ranking for the first time on july, 8, 2002. at the age of 16, she played against karsten braasch the battle of sexes. she was the highest paid female athlete in 2016. she is a unicef ambassador .