What concerns did colonists have related to representation in parliament?

No, the American colonies did not have representation in Parliament. But you say, As the colonies are not represented in the British Parliament, they are entitled to a free power of legislation. For they inherit all the right which their ancestors had of enjoying all the privileges of Englishmen.

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The correct answe is that colonist were afraid that the members of parlament would not look out for their interest.

Explanation:

This is when the famous phrase "No taxation without representation" began as the colonist felt that government should not make choice for them if did not represent their interst.

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