This cartoon was published by benjamin franklin in 1754. given the date, it was most likely used for the first time prior to which conflict?

French and Indian War

Explanation:

The French and Indian War was the American phase of a global nine years’ conflict (1754–63) between France and Great Britain.

This dispute settled the control over the large colonial region of North America. There were three earlier phases of this extended struggle for overseas control, including King William’s War (1689–97), Queen Anne’s War (1702–13), and King George’s War (1744–48).

Franklin´s cartoon was meant to express the need for the colonies to join the Kingdom of Great Britain to overcome the French and Indians.