What are the three limitations of an ELISA

Enzyme-linked Immunosorbent Assay, also know as ELISA are used to determine if an antibody is present in a patient's blood sample. They are found in bodily fluids.

Explanation:

The three important limitations are:

1) A positive result confirming a presence of an antibody but it not necessarily making the patient sick

2) A false negative where the amount of antibodies is too low to be measured

3) Another positive result may occur if an unrelated antibody reacts with the antigen non specifically.

answer; i believe the correct answer is ( mediation);

can you plz give me the following