The geographic isolation of two populations of a species tends to increase differences between their gene pools because it

a. prevents interbreeding between the populations.

b. prevents interbreeding within each population.

c. causes temporal isolation of the two populations.

d. increases differences in courtship behavior.

The best and most correct answer among the choices provided by your question is the first choice or letter A.

The geographic isolation of two populations of a species tends to increase differences between their gene pools because it prevents interbreeding between the populations. 

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The answer is    d.  central highlands