Santiago is writing a research-based informative essay on how discarded plastic affects the ecosystems of North America’s oceans. Santiago has analyzed relevant sources and organized information on note cards. Through his research, he has developed multiple research questions. Which question should Santiago ask himself to continue this research process?

Should I use more online tools to locate more useful information?
Which research question is the most important one for me to focus on?
Should I gather additional primary sources to confirm my findings?
Which research question would my audience most enjoy learning about?