8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Aplate clutch has a single pair of mating friction surfaces 250-mm od by 175-mm id. the mean value of the coefficient of friction is 0.30, and the actuating force is 4 kn. a) find the maximum pressure and the torque capacity using the uniform-wear model. b) find the maximum pressure and the torque capacity using the uniform-pressure model.

Water at 55c flows across a flat plate whose surface temperature is held constant at 95c. if the temperature gradient at the plate's surface for a given value of x is 18 c/mm, find a) local heat transfer coefficient. b) heat flux

Steel balls 12 mm in diameter are annealed by heating to 1150 k and then slowly cooled to 400 k in an air att 325 k and h -20 w/m2 k. assume the properties of the steel to be k- 40 w/m k,p 7800 kg/m3, and cp 600 j/kg k. a. determine whether lumped system method can be used to analyze this problem and if so why" b. determine the time required for the cooling process. c. determine the total amount of heat lost from each ball to the ambient air