Method development 25-27.

(a) explain how to measure k and resolution.

(b) state three methods for measuring im in reversed-phase chroma- tography

(c) state three methods for measuring om in hydrophilic interaction liquid chromatography.

(d) estimate tm for a 15 x 0.46 cm column containing 5-um parti- cles operating at a flow rate of 1.5 ml/min.

estimate tm if the par- ticle size were 3.5 um instead.