The data below were collected for an enzyme-catalyzed reaction. determine the vmax and km for this reaction

The parameters vmax and km are determined using known models in enzyme reactions. One linear model in enzyme reactions is the Lineweaver-Burke model. Its form is written as

1/v = (Km/vmax)(1/S) + 1/vmax,
where
Km/vmax is the slope
1/vmax is the y-intercept

In order to determine the parameters, plot (1/v) vs (1/S) data, where v is the rate of reaction and S is the substrate concentration. Then, find the y-intercept which is the point at which the line intersects the y-axis. The reciprocal of the y-intercept is equivalent to vmax. For Km, find the slope through linear regression. Then, multiple vmax to the slope to determine Km.