Why is the same type of nail used in all test tubes? in every experiment done to show how rusting works and in what conditions.

The independent variable is the type of music.the dependent variable is the pulse rate.the intervening variable is the age of the subject.if the mother does not like rock, her pulse is likely to not increase.the same might be true for the sister.the friend may like rap and gets excited.to establish cause/effect relationship, all of the subjects must be consistent.hope i .