Limited ATP Synthesis in the Dark in a laboratory experiment, spinach chloroplasts are illuminated in the absence of ADP and Pi, then the light is turned off and ADP and Pi are added. ATP is synthesized for a short time in the dark. Which of the following is the most reasonable explanation? A. The illumination has led to establishing an electron gradient. When ADP and Piare added, ATP synthesis is driven by the electrons, which becomes exhausted in the absence of light.
B. During illumination, H2O is reduced to 02, which causes the establishment of an electron gradient. When ADP and Pi are added, ATP synthesis is driven by the gradient, which becomes exhausted in the absence of light
C. During illumination, a proton gradient is established. When ADP and Pi are added, ATP synthesis is driven by the gradient, which becomes exhausted in the absence of light.
D. When there is no light there will be energy because a bond will form between ADP. Pl and make ATP.