Using your freshly made 5M stock aqueous solution of NaCl and your 10% aqueous stock of SDS, you find a prepackaged bottle of 1M Tris-Hcl (aqueous solution) along with a 95% solution of liquid NP-40 in a bottle. Using these stock reagents calculate the amount needed for the following final concentrations for each reagent as well as the final volume of water needed to obtain: Total Final volume of 500 mL
Final Concentrations:
150 mM NaCl
50 mM Tris-HCl
1% NP-40 .
1% SDS