For the wild type (unmutated) enzyme, you measure a rate of p-nitrophenol release by the change in absorbance at 405 nm (for the slower phase only) of 0.216 (in a cuvette with a pathlength of 1 cm) over the course of two minutes. Use the Beer-Lambert Law to calculate the concentration of product released over this time; the extinction coefficient of p-nitrophenol at 405 nm is 18,000 M-1 cm-1.

1.2x10⁻⁵M = Concentration of the product released

Explanation:

Lambert-Beer's law states the absorbance of a solution is directly proportional to its concentration. The equation is:

A = E*b*C

Where A is the absotbance of the solution: 0.216

E is the extinction coefficient = 18000M⁻¹cm⁻¹

b is patelength = 1cm

C is concentration of the solution

Replacing:

0.216 = 18000M⁻¹cm⁻¹*1cm*C

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