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Imagine that you have produced several versions of lactase, each of which differs from normal lactase by a single amino acid. describe a test that could indirectly determine which of the versions significantly alters the three-dimensional shape of the lactase protein.

Butadiene undergoes a reaction at a certain temperature in the gas phase as follows: 2c4h6(g) --> c8h12(g) the following data were collected for this reaction: time (min) [c4h6] (m) 0 0.36 15 0.30 30 0.25 48 0.19 75 0. determine the order of the reaction and the rate constant. 1st order and k = 4.3x10 -4 s-1 1st order and k = 2.3x10-4 s-1 2nd order and k = 4.3x10-4 s-1 2nd order and k = 2.3x10-4 s-1 zero and k = 4.3x10-4 s-1

List four observations that indicate that a chemical reaction may be taking place