Imagine you followed all steps correctly, with multiple attempts, and found that the purity of recombinant DHFR isolated through IMAC was not good enough. Hypothesize two changes to the procedure or experimental design that you could consider trying next to increase the purity of our DHFR sample and why you anticipate each to increase protein purity. (5pt)

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Explanation:

the mantle

explanation:

earth is divided into three layers: the crust or the outermost layer on which we live; the underlying, middle layer - the mantle - and the core (the innermost layer of earth).

it should have an internal chamber and two thermometers sticking out of the top. the internal chamber will also be closed off from the outside.