Other than solubility of sample in the solvent, what is the criteria for the solvent (other than cheap and nontoxic) used for preparing the sample that impacts the experiment and why would this criteria be important in the spotting phase if you have to make repeated applications of the sample?

Answer and Explanation:

In order for the spotting phase to be efficient and for the sample to register the repeated applications of the sample, it is necessary that in addition to the solubility and low toxicity, the solvent has a low boiling point, but that allows an efficient and rapid evaporation, whenever necessary, so that it is possible to release the sample on the TLC plate.