You have a 5 kb vector and a 1 kb PCR fragment in separate solutions. You load 5 microliters of each into separate lanes on an agarose gel, and you compare the intensities by gel staining. The two bands appear to be of equal intensity. What volumes of vector and fragment would you add to a ligation reaction to have a 5:1 molar ratio of fragment to vector?

nacl or sodium chloride is an ionic compound. this is because an ionic compound pertains to a compound that consists of positively and negatively charged ions. in this case, na (sodium) has a tendency to lose one electron when it reacts with with other atoms, so the resulting sodium ion contains 11 protons and 10 electrons, thus making it positively charged (na+), when chlorine reacts, it usually gains one electron to form an ion called chloride, thus making it negatively charged ( since the ions are oppositely charged, they attract.