Most suitable method for isolation and purification of proteins having same molecular weight/size?

Spontaneity tell us the direction of the reaction, but not how fast it goes. gibbs free energy brings together enthalpy, entropy and temperature in one easy formula. g = h - ts. and, finally the sign of delta g is always negative for a spontaneous reaction.

D=m/v =57.9/3 = 19.3 g/cm^3

She was of necessity running a grass roots target audience was unlettered