What concentration of monosodium phosphate would be required to make a solution of pH 7.4 with 0.2 M disodium phosphate ( pKa

The concentration of monosodium phosphate is 0.1262M

Explanation:

The buffer of H₂PO₄⁻ / HPO₄²⁻ (Monobasic phosphate and dibasic phosphate has a pKa of 7.2

To determine the pH you must use Henderson-Hasselbalch equation:

pH = pKa + log [A⁻] / [HA]

Where [A⁻] is molarity of the conjugate base of the weak acid, [HA].

For H₂PO₄⁻ / HPO₄⁻ buffer:

pH = 7.2 + log [HPO₄⁻² ] / [H₂PO₄⁻]

As molarity of the dibasic phosphate is 0.2M and you want a pH of 7.4:

7.4 = 7.2 + log [0.2] / [H₂PO₄⁻]

0.2 = log [0.2] / [H₂PO₄⁻]

1.58489 = [0.2] / [H₂PO₄⁻]

[H₂PO₄⁻] = 0.1262M

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