During the isolation of Bacterial DNA, the mixture (Cell debris, genetic material, and proteins in buffers) is transfered to a centrifuge tube and treated with an equal volume of chloroform:isoamyl alcohol (24:1 v/v) solution. This is then manually shaked for 15 minutes and centrifuged for 10 minutes at 3000rpm. Following centrifugation, 3 layers are observed in the tube; name the layers accordingly and state what is contained within them:

fr8t7iot7o87oglgt87ot79p0[90

a. the law of proportion states the combination of 2 atoms in a specific ratio to produce multiple compounds such as the given statement of question ‘1st compound contains 88.8 % cu ’ indicates 88.8 g of cu is present along with 11.2 g oxygen in 100 g of compound whereas the statement ‘2st compound contains 79.9 % cu ’ indicates 79.9 g of cu is present along with 20.1 g oxygen in 100 g of compound

1st compound contains 88.8 g cu

for cu

m = 63 g /mol

atomic % = 88.8 /63 = 1.42

for o

m = 16 g /mol

atomic % = 11.2 /16 = 0.7

b. the emperical formula of 1st compound is

cu1.4o0.7

cu2o