The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that also contains 300mM imidazole. Describe how the instructor made the 0.25L elution buffer for all the students this week given 500ml of 1M of Tris (121.1 g/mole) (pH8.0), 750ml of 5M NaCl (MW

When would a bouncy ball have the most potential energy

Many reactions take place in aqueous solution. when potential reactants are mixed, a reaction will occur if there is some driving force that favors the formation of products. it is often convenient to categorize reactions in terms of these driving forces: precipitate formation, in which an insoluble solid is formed, weak electrolyte formation, as in a neutralization reaction involving water, or transfer of electrons, as in a redox reaction. these reactions can be represented by full molecular equations, which contain all species in the reaction mixture, or by net ionic equations, which show only the species that actually undergo a change. the latter does not contain the spectator ions, which do not undergo a net change or do not take part in the reaction. part a when the following two solutions are mixed: k2co3(aq)+fe(no3)3(aq) the mixture contains the ions listed below. sort these species into spectator ions and ions that react. drag the appropriate items to their respective bins. view available hint(s) spectator ions ions that react part b what is the correct net ionic equation, including all coefficients, charges, and phases, for the following set of reactants? assume that the contribution of protons from h2so4 is near 100 %.ba(oh)2(aq)+h2so4(aq)→ express your answer as a chemical equation. view available hint(s) nothing provide feedback

What is the relationship between wind and ocean waves? question 17 options: wind moving at higher speeds will transfer more energy to the water, resulting in stronger waves. wind moving at higher speeds will transfer energy over a larger part of the ocean water, resulting in waves with a shorter wavelength. winds moving at higher speeds with cause water to move forward at faster rates, causing larger ocean waves. winds moving at higher speeds will affect deeper water, resulting in waves that move at a faster rate. how do temperature and salinity affect deepwater currents? question 15 options: as temperatures and salinity levels of water increase, the water rises to the surface where it creates currents as it moves to colder regions. they create changes in wind direction, moving denser water in the same direction as the wind and causing the deepwater circulation patterns found in the ocean. they equalize the forces on undersea currents caused by the coriolis effect as they replace more dense water with less dense water. they create density differences that cause dense deepwater currents to flow toward the equator where they displace less dense, warmer water above them.