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Chemistry, 21.04.2020 20:52 alexcuevaz90

To survive in an acidic environment, the cells utilize the energy of ATP hydrolysis to pump out 2 protons against its electrochemical potential. Calculate the minimum level of cellular ATP (in unit of uM) necessary for this operation. The cellular concentrations of ADP and Pi are constant at 38 uM and 120 uM, respectively. The pH inside the cell is 7, and the cell is placed in a culture solution having a pH of 5.8. The standard free energy of ATP hydrolysis is -31.3 kJ at 37oC (Biochemistry standard state). The membrane potential is -0.07 V.

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