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The retention time (tr) and peak width (w) for compounds A and B are given after chromatographic separation using five different experimental parameters. Which chromatographic separations achieved a resolution that is desirable for quantitative analysis?
a. Separation 1: compound A: t; = 13.0 min; w = 5.0 min compound B: t; = 17.0 min; w = 6.5 min
b. Separation 3: compound A: tr = 14.0 min; w = 4.0 min
c. Separation 2: compound A: t; = 10.0 min; w = 3.0 min compound B: t; = 19.0 min; w = 3.0 min
d. Separation 4: compound A: tr = 11.0 min; w = 2.0 min compound B: tr = 16.0 min; w = 5.0 min compound B: tr = 15.0 min; w = 3.0 min
e. Separation 5: compound A: tr = 9.0 min; w = 2.5 min compound B: t; = 13.0 min; w= 4.5 min
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