Exactly 5.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 137 ppm of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.00, 5.00, 10.00, 15.00, and 20.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, which gave values of 126.0, 290.4, 454.8, 619.2, and 783.6, respectively. What is the concentration (in ppm) of phenobarbital in the unknown?
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Exactly 5.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetr...
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