Which of the following statements about the oxyanion hole in serine proteases are true? This preformed loop is properly positioned to stabilize the negatively charged tetrahedral intermediate involved in amide bond hydrolysis. The backbone NH bonds of S195 and G193 create H-bonding interactions with the developing negative charge as S195 attacks the scissile carbon bond. It represents an enthalpic interaction that helps stabilize the transition state. It represents an electrostatic interaction that leads to a lower energy