Two DNA sequences, each 48 kb in size, are treated with the same restriction enzyme. There is only one site for this restriction enzyme on each of these sequences. Digests are separated by gel electrophoresis. Sequence A shows ONE band that is 48 kb in size. Sequence B shows TWO bands, one 30 kb and the other 18 kb in size. What can you conclude from these data?A. Sequence A is linear, sequence B is circular B. Both sequences are linear. >X C. They are identical sequencesD. Sequence A is circular, sequence B is linear 0

Sequence A is circular, sequence B is linear.

Explanation:

The restriction enzymes are known as molecular scissors because they cuts the DNA molecules and generator the desired DNA fragment. Two main types of restriction enzymes are exonuclease and endonuclease.

The Genome map can be constructed by the using the restriction enzyme. The circular DNA gives the DNA fragments equal to the digesting site of the restriction enzyme whereas linear DNA give fragments one more than the restriction site of the enzyme. In the given question, Sequence A has one fragment only and Sequence B has two fragments.

Thus, the correct answer is option (D).