In 1906, harden and young, in a series of classic studies on the fermentation of glucose to ethanol and co2 by extracts of brewer's yeast, made the observations inorganic phosphate was essential to fermentation; when the supply of phosphate was exhausted, fermentation ceased before all the glucose was used; during fermentation under these conditions, ethanol, co2, and a sugar phosphate accumulated; when arsenate was substituted for phosphate, no sugar phosphate accumulated, but the fermentation proceeded until all the glucose was converted to ethanol and co2. which enzyme of glycolysis requires inorganic phosphate and, therefore, stops when no phosphate is available? phosphoglycerate mutase hexokinase phosphoglycerate kinase glyceraldehyde 3‑phosphate dehydrogenase what sugar phosphate accumulates under these conditions? glucose 1‑phosphate fructose 1,6‑bisphosphate fructose 1‑phosphate glucose 1,6‑bisphosphate arsenate substitution for phosphate generated an acyl arsenate compound that immediately degraded. what glycolysis intermediate was a product of the spontaneous degradation of this acyl arsenate? 3‑phosphoglycerate glyceraldehyde 3‑phosphate dihydroxyacetone phosphate glycerol 3‑phosphate