Asample containing anthracene dissolved in methane was separated using gas chromatography on a column with mobile phase ) and stationary phase ) volumes of 24.7 ml and 9.88 ml, respectively. for this particular column, anthracene has a retention volume ) of 54.53 ml. calculate the retention factor and the partition coefficient (k) for anthracene in this separation.

Explanation:

Formula to calculate retention factor is as follows.

               Retention factor (k) =

where,     = adjusted retention volume =

              = hold up volume

              = total retention volume

Therefore, putting the given values into the above formula as follows.

      Retention factor (k) =

or,  Retention factor (k) =

                                      =

                                      = 1.21

Now, partition coefficient (K) will be calculated as follows.

                k =

                     =

                     = 3.025

Thus, we can conclude that the value of retention factor is 1.21 and partition coefficient (K) is 3.025.

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