You perform a ligation with ncoi/noti-digested, pet-41a vector and the egfp insert from pegfp-n1 (as in lab). you then transform the ligation mix into e. coli and plate on lb medium containing kanamycin. consider the following unexpected outcomes, and suggest controls: nothing grows. what controls might you design to determine whether the e. coli cells are viable (alive) versus whether the e. coli is competent? what would the results of those controls be? you see lots of growth, but when you isolate plasmid from numerous e. coli colonies, all you find is pet-41a with no insert. suggest the most likely way these colonies arose (aside from contamination). what would the results of your controls look like if your suspicion was correct?