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Chemistry, 04.10.2019 20:10 risarod6106

Interestingly, the quantum yield for nadh increases dramatically when it is bound to an enzyme compared to when it is free in solution (φf = 0.1 vs 0.02, respectively) . a. assume that a pair of solutions contain the same concentration of nadh, in different forms: i. solution 1: 100% free in solution ii. solution 2: 50% bound to an enzyme; remainder is free. you can assume that the enzyme itself has negligible fluorescence. how much brighter is solution 2 than solution 1, in terms of fluorescent intensity?

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