Inhibitors of mitochondrial function have provided extremely useful tools for analyzing how mitochondria work. below are shown three distinct patterns of oxygen electrode traces obtained using a variety of inhibitors. in all the experiments mitochondria were added to a phosphatebuffered solution containing succinate as the sole source of electrons for the respiratory chain. after a short interval adp and then an inhibitor were added, as indicated below. the rates of oxygen consumption at various times during the experiment are shown by downward sloping lines, with faster rates shown by steeper lines. a). from the description of the inhibitors in the list that follows, assign each inhibitor to one of the oxygen traces above. all these inhibitors stop atp synthesis. note: several inhibitors can produce the same profile. give a brief explanation in each case. (12 pts)
a). from the description of the inhibitors in the list that follows, assign each inhibitor to one of the oxygen traces above. all these inhibitors stop atp synthesis. note: several inhibitors can produce the same profile. give a brief explanation in each case. (12 pts) inhibitor function 1 fccp make membranes permeable to protons 2 malonate prevent oxidation of succinate 3 cyanide inhibit cytochrome oxidase 4 atractylate inhibit the adp-atp antiporter 5 oligomycin inhibit atp synthase 6 butylmalonate block mitochondrial uptake of succinate b). using the same experimental protocol as above, describe what changes in oxygen consumption you would expect from the sequential addition of the pairs of inhibitors in the list below. (6 pts) give a brief explanation in each case. b1. fccp followed by cyanide b2. fccp followed by oligomycin b3. oligomycin followed by fccp
2. calculate the minimum value of ∆e'°o that must be generated by a pair of electron carriers to provide sufficient energy for atp synthesis. assume that a pair of electrons is transferred. note that the faraday constant f = 23.06 kcal/v*mol.