Chemistry, 09.07.2019 21:30 cece3467

Protein x has an absorptivity of 0.4 ml·mg-1 ·cm-1 at 280 nm. what is the absorbance at 280 nm of a 2.0 mg ·ml-1 solution of protein x? (assume the light path is 1cm).

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Answer from: croxy0514

Absorbance measures the ability of the substance to absorb light at a specific wavelength.

Absorbance is also equal to the product of molar absorptivity, path length and molar concentration.

The mathematical expression is given as:

$A= \epsilon l c$ (1)

where, A = absorbance

$\epsilon$ = molar absorptivity

l = path length

c = molar concentration.

The above formula is said to Beer's Law.

Absorptivity of protein x = $0.4 mLmg^{-1}cm^{-1}$

Path length = 1 cm

Molar concentration = $2.0 mg mL^{-1}$

Put the values in formula (1)

$Absorbance at 280 nm = 0.4 mL mg^{-1}cm^{-1}\times 1 cm \times 2.0 mg mL^{-1}$

= 0.8

Thus, absorbance at 280 nm = 0.8

Answer from: Quest

the tides are influenced by both the moon and the sun, it's easy to see that when the sun lines up with the moon and the earth, as during a new moon or full moon, the tidal effect is increased. these are known as spring tides, named not for the season, but for the fact that the water "springs" higher than normal. on the other hand, if the sun and the moon are 90 degrees apart in relation to an observer on earth as during the first quarter moon or third quarter moon, then high tides are not as high as they normally would be. this is because despite its greater distance, the sun's mass allows it to exert enough gravitational force on the oceans that it can negate some of the effects of the moon's pull. this phenomenon of lower high tides is called a neap tide. if you need more information on this topic visit moonconnection.com.

Answer from: Quest

explanation:

let the different atoms be x and y. each with four protons and same mass will be represented as follows.

mass number is number of protons + number of neutrons.

$^{m}_{4}x$ and $^{m}_4y$

since, it is given that one has a negative charge while the other is neutral. therefore, there will be increase in number of protons and the atom will be represented as follows.

$^{m}_{5}x^{-}$

according to periodic table, mass number of beryllium is 9 and it has atomic number 4 so, the atoms will be $^{9}_{5}be^{-}$ and $^{9}_{4}be$ .

the protons and neutrons will be located inside the nucleus whereas the electrons will be revolving around the nucleus.

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Protein x has an absorptivity of 0.4 ml·mg-1 ·cm-1 at 280 nm. what is the absorbance at 280 nm of a...

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