How many variables are tested in any controlled experiment?

The phylogenetic tree illustrates the relationship between humans and our closest living relatives. the tree was based on biochemical comparisons, including dna and amino acid sequences. according to the biomolecular data, we could infer that a) the four organisms do not have a common ancestor. b) humans are more closely related to chimps than any other apes. c) chimps are more closely related to gorillas than they are to humans. eliminate d) there is no evidence if any relationship between the four branches on the tree.

Factors that can increase mutation rates a. high temps b. low temps c. food additives d. uv rays

In general, are there any major differences that you can see? explain