Why was it necessary to use only Klenow fragment, rather than intact E. coli Pol I, in DNA sequencing reactions?

i won't lie i don't know so go online and ask google thats what i do

linnaeus is the father of taxonomy, which is the system of classifying and naming organisms. one of his contributions was the development of a hierarchical system of classification of nature. today, this system includes eight taxa: domain, kingdom, phylum, class, order, family, genus, and species.