How do we know a particular lineage of prokaryote exists in a natural habitat without first isolating and growing it in laboratory culture

Which table best shows the impacts resulting from human activity? impact of human activities human activity impact forest area cleared to construct an airport increase in oxygen level vegetation cleared to construct an oil mine increase in pollution impact of human activities human activity impact forest area cleared to construct an airport increase in sound pollution vegetation cleared to construct a power plant contamination of water impact of human activities human activity impact beehives cleared from urban areas increase in vegetation dams constructed on a river increase in fish population impact of human activities human activity impact beehives cleared from urban areas increase in bird population dams constructed on a river better quality of water

Transmission electron microscope are best for viewing a) microorganisms in pond water b) internal organs of a mouse c)internal structures of a cell d) surface features of a specimen

Is there any species that went extinct in recent years due to natural causes (not caused by human interaction). if so, what caused it?