The β chains of HbA and HbS were treated with trypsin, and the sequence of the N-terminal tryptic peptides are as given below. Do these peptides separate from each other in an electric field if the pH is 7.0? Explain in detail the reasoning behind your answer and include your calculations for the charge of each peptide in your answer.
HbA: Val-His-Leu-Thr-Pro-Glu-Glu-Lys
HbS: Val-His-Leu-Thr-Pro-Val-Glu-Lys
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