In two-dimensional electrophoresis Question 3 options: Proteins are separated by native size with molecular sieve electrophoresis. Then the proteins are separated by their isoelectric point with isoelectric focusing. Denatured proteins are separated by subunit size with denaturing SDS electrophoresis. Then native proteins are separated by their isoelectric point with isoelectric focusing. Native proteins are separated by their isoelectric point with isoelectric focusing. Then they are separated by subunit size with denaturing SDS electrophoresis. Native proteins are separated by their charge with isoelectric focusing. Then they are separated by protein size by denaturing SDS electrophoresis. Native proteins are separated by protein size with SDS electrophoresis. Then denatured proteins are separated by their charge with isoelectric focusing.